组织在制作过程中,由于化学试剂的作用封闭了抗原,又由于热的作用致使部分抗原的肽链发生扭曲,致使在免疫组化的染色过程中不能将其显示出来,为了解决上述的问题,利用化学试剂和热的作用将这些抗原重新暴露出来或修正过来的过程称为抗原修复。
文章详细信息 关键词: 全自动免疫组化染色仪;;人工操作;;免疫组化;;抗原修复 [gap=778]eywords: immunohistochemistry autostainer;manual operation;immunohistochemistry;antigen repair
基于12个网页-相关网页
Antigen retrieval, organization background closed, dropping a specific antibody.
抗原修复,组织背景封闭,滴加特异性抗体1。
参考来源 - 肾内源性干细胞在急性肾小管坏死修复中的作用·2,447,543篇论文数据,部分数据来源于NoteExpress
结论:石蜡切片经系列抗原修复后免疫反应有一定改善,但与应用的抗体类型有关。
Conclusion: the IR on the paraffin section could be improved after treatment with a series of the tissue antigen recovery, and the effect may be concerned with different kinds of antibodies.
结论用枸橼酸缓冲液(PH6.0)微波抗原修复组织样本及PV检测法是CD 117免疫组化表达的较为理想的检测方法。
Conclusions it is the ideal method of detection of CD117 antigen that CD117 antigen was retrieved by citric acid buffer (PH6.0) microwave antigen retrieval and detected by PV staining.
结果石蜡组织切片免疫组化反应预处理,采用中温水浴抗原修复法,其阳性表达率为5 2 .6% ,脱片率为2 .0 % ;
Results Prerteat organization chip, using the middle temperature water bath antigen repairing method, the positive expressing rate is 52.6% and the unfalling rate is 2.0%;
应用推荐