结论:石蜡切片经系列抗原修复后免疫反应有一定改善,但与应用的抗体类型有关。
Conclusion: the IR on the paraffin section could be improved after treatment with a series of the tissue antigen recovery, and the effect may be concerned with different kinds of antibodies.
结论用枸橼酸缓冲液(PH6.0)微波抗原修复组织样本及PV检测法是CD 117免疫组化表达的较为理想的检测方法。
Conclusions it is the ideal method of detection of CD117 antigen that CD117 antigen was retrieved by citric acid buffer (PH6.0) microwave antigen retrieval and detected by PV staining.
结果石蜡组织切片免疫组化反应预处理,采用中温水浴抗原修复法,其阳性表达率为5 2 .6% ,脱片率为2 .0 % ;
Results Prerteat organization chip, using the middle temperature water bath antigen repairing method, the positive expressing rate is 52.6% and the unfalling rate is 2.0%;
方法用免疫组化SABC法检测五种单克隆抗体,分别使用水煮法、微波法、高压法修复抗原,从中选出最佳修复方法。
Methods Check five kinds of antibody with immunohistochemistry SABC method and chose the best antigen retrieval method among poach method, microwave method and high pressure method respectively.
目的:探讨经抗原系列修复后冰冻切片抗体应用于石蜡切片的免疫组化技术。
Aim: to explore how to make the antibody which is originally applied to the frozen section be used for the paraffin section through a series of treatments of the tissue antigen recovery.
组织需要进行水浴抗原热修复;
而在创面组织修复过程中,糖尿病创面增殖细胞核抗原,P 53蛋白表达明显少于非糖尿病创面。
But during the wound repairing, much less expression of PCNA and P53 protein in diabetic skin tissue compared with non-diabetic skin tissue.
目的研究去抗原异种松质骨支架材料的生物相容性,为其在骨缺损修复领域中的临床应用提供实验依据。
Objective To study the biocompatibility of antigen-extracted xenogeneic cancellous bone matrix for the clinical use in the bone reconstruction field.
目的研究去抗原异种松质骨支架材料的生物相容性,为其在骨缺损修复领域中的临床应用提供实验依据。
Objective To study the biocompatibility of antigen-extracted xenogeneic cancellous bone matrix for the clinical use in the bone reconstruction field.
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