结果石蜡组织切片免疫组化反应预处理,采用中温水浴抗原修复法,其阳性表达率为5 2 .6% ,脱片率为2 .0 % ;
Results Prerteat organization chip, using the middle temperature water bath antigen repairing method, the positive expressing rate is 52.6% and the unfalling rate is 2.0%;
结论用枸橼酸缓冲液(PH6.0)微波抗原修复组织样本及PV检测法是CD 117免疫组化表达的较为理想的检测方法。
Conclusions it is the ideal method of detection of CD117 antigen that CD117 antigen was retrieved by citric acid buffer (PH6.0) microwave antigen retrieval and detected by PV staining.
方法用免疫组化SABC法检测五种单克隆抗体,分别使用水煮法、微波法、高压法修复抗原,从中选出最佳修复方法。
Methods Check five kinds of antibody with immunohistochemistry SABC method and chose the best antigen retrieval method among poach method, microwave method and high pressure method respectively.
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