If this reforms, so the plasmid reforms back to its native state, that resistance will be recovered.
如果它自己合上了,质粒就会回到原本的状态,抗性就会恢复
This process of using a biological event like resistance to Ampicillin in order to pick out the cell population that you're interested in is called selection.
这个利用生物特性,如对氨苄青霉素的抗性,来挑出你所感兴趣的细胞的过程叫筛选
But now, this is the piece de resistance.
但是现在,这变成了阻碍。
Now if all we have to go on is just this semiotic system, it's going to be kind of hard to put up resistance to by the same token we'll say "yellow" means "pause," "green" means "go."
而从语义学角度分析,我们必须不能这么想,这样绿色自然就表示继续,黄色表示暂停“
But, De Gaulle perpetuated this myth about the resistance.
但是,戴高乐延续了这个关于抵抗的传说
If you selected the cells right than the only ones that have that resistance to Ampicillin are the ones that successfully got your plasmid and are using this Ampicillin resistant gene.
如果你选对了细胞,那么对氨苄青霉素有抗性的就是,成功得到了质粒并表达了基因的细胞
If we grew these cells on a plate loaded with Ampicillin and we could select cells that have Ampicillin resistance, and this process of selection and cell culture is very important and we'll talk about it more next week.
如果我们在含有氨苄青霉素的,培养基上培养细胞,我们可以筛选出,对氨苄青霉素有抗性的细胞,这个筛选和培养细胞的过程是很重要的,我们下星期会讲到这些
应用推荐