Sequencing analysis shows that the CpTI gene has been modified as what was DE - signed.
序列分析表明了基因修饰的准确性。
MethodsThe corresponding designed primers, RT_PCR, gene cloning, DNA sequencing analysis were used.
方法设计相应的引物，用RT _ PCR，基因克隆，DNA序列分析技术。
Ampicilin - resistant transformants were selected and identified by PCR, Enzyme digestion and DNA sequencing analysis.
并对氨苄筛选的重组入外源基因的质粒通过P CR、酶切和测序鉴定。
DNA sequencing analysis proved that the sequence ofthe radiation-inducible promoter was totally consistent with the designing sequence.
合成启动子序列分析与设计序列完全一致；
Conclusion MTC can be diagnosed at gene level by direct gene sequencing analysis. It is possible to diagnose MTC before operation by means of molecular genetic analysis.
结论直接基因测序分析能在基因水平诊断MTC，分子遗传学分析使术前诊断该疾病成为可能。
While the WASP gene sequencing analysis could confirm the diagnosis find carriers and provide the evidences for hematopoietic stem cell transplantation treatment in WAS patients.
WASP基因分析可确诊患者，发现携带者，为干细胞移植治疗患者提供依据。
By PCR-SSCP analysis, 17 PCR products were identified with different mobility of single strand DNA in propositus. 9 suspectable mutations were revealed with DNA sequencing analysis.
SSCP分析发现17个外显子pcr产物单链dna迁移率异常，通过DNA直接测序发现9个外显子可疑突变，但反向测序均未能证实。
Methods immunohistochemistry in 52 cases of nasopharyngeal carcinoma PTEN gene expression, the use of PCR SSCP silver staining, DNA sequencing analysis to detect PTEN gene mutation in exon 5, 8.
方法采用免疫组化检测52例鼻咽癌中pten基因的表达，利用PCRSSCP银染、DNA测序分析等方法检测pten基因第5、8外显子突变。
Additional laboratory analysis, including gene sequencing, is ongoing.
目前正在进行其它实验室分析，包括基因测序。
The technology for exome sequencing is also further along than for whole-genome sequencing, Wang added, including not only the sequencing, but the capture technologies and the data analysis.
外显子组测序技术也比全基因组测序走得更远，Wang补充道，不仅仅是测序，还包括捕获技术和数据分析。
Gene sequencing and homology analysis show that these novel enzymes share remarkable homology with the amylase family.
基因测序及同源性分析表明这些新酶与淀粉酶家族具有很强的同源性。
The popularity of rapid DNA sequencing technology provides large amounts of data for molecular phylogenetics, while the sequence analysis technology is the important tool to gain knowledge from data.
DNA快速测序技术的普及为分子系统学家提供了大量数据，而序列分析技术则是探索数据发现知识的重要工具。
DNA microarray has been applied to DNA sequencing, pharmaceuticals analysis, gene expression and so on.
DNA微集阵列已广泛用于DNA测序、药物分析、基因表达等研究领域。
After the completion of human genome sequencing, biologists desire for higher processing and analysis power to handle the huge gene data.
人类基因组测序工作完成后，对基因数据的处理和分析能力提出了更高的要求。
After the completion of human genome sequencing, the biologists require higher processing and analysis power to handle the huge gene data.
人类基因组测序工作完成后，对基因数据的处理和分析能力提出了更高的要求。
Methods We used heteroduplex mobility analysis (HMA) and sequencing to detect mixed infection.
方法使用异双体动态分析法和测序检测混合感染。
Analysis of next generation sequencing data present a formidable computational challenge.
下一代测序数据的分析提出了一个强大的计算挑战。
Advances in sequencing technologies pose challenges for high-throughput analysis of large-scale sequence data.
测序技术的进展为大规模序列数据的高通量分析提出了挑战。
Mutation analysis was detected by direct sequencing RT-PCR products.
直接测序法检测RT PCR产物的基因变异；
In addition to supporting conventional applications for PCR, hybridization and sequencing, NoePrimer provides advanced capabilities for multiplex PCR and high-throughput primer search and analysis.
它不但能进行常规PCR引物、杂交探针和测序引物的设计，同时，它还能进行多重pcr引物分析以及高通量的引物搜索和查找功能。
BACKGROUND: Due to the advanced techniques in sequencing and fragment analysis, DNA sequencers and analyzers produce vast amounts of data within short time.
背景：由于测序和片段分析中的先进技术，DNA测序仪和分析仪在短时间内就产生了大量的数据。
Simultaneously the computational analysis of the large volumes of data generated by the new sequencing machines remains a challenge.
同时，由新的测序机器产生的大量数据的计算分析仍然是一个挑战。
And identified HEV71 isolates were performed by gene amplification of VP1 coding region, nucleotide sequencing and homology analysis of evolution.
对分离到的HEV71阳性分离株进行VP1编码区基因扩增，核苷酸序列测定和同源进化分析。
However, at present, the analysis of next-generation sequencing data is challenging, in particular because most sequencing platforms provide short reads, which are difficult to align and assemble.
然而，目前，下一代测序数据的分析仍然面临挑战，尤其是大多数测序平台产生了短序列，这对比对和组装造成了困难。
The purified DNA can be used directly in subsequent enzymatic reactions such as labeling, sequencing, cloning, and ligation, as well as for PCR analysis.
纯化的DNA可直接用于后续的应用，如标记、测序、克隆、连接和PCR分析。
Specific primers were designed for MSX1 and PAX9 respectively. Mutation analysis was performed by direct sequencing of all the coding exons and intron-exon boundaries.
分别设计MSX1、PAX9基因特异性引物，聚合酶链反应扩增全部外显子编码区和内含子-外显子剪接序列，产物纯化后直接测序。
Analysis of RT-PCR and sequencing indicated that the ncRNA gene had two different transcripts through alternatively splicing pattern.
PCR和测序结果分析，发现这个非编码RNA基因通过选择性剪接产生两种不同的转录产物。
Mapping the sequencing reads to reference genomes efficiently and effectively is one of the most critical analysis tasks.
高效且有效地比对测序片段到参考基因组上是最关键的分析任务之一。
Conclusions: The new high resolution melting curve analysis is more efficient, more convenient than direct sequencing. Moreover, it is a low-cost test, which is suitable for clinic test.
结论：新的高分辨率熔解曲线分析法在检测KRAS基因突变时比直接测序法具有耗时短，易操作的优点，并且检测成本低廉，适合在临床开展。
Conclusions: The new high resolution melting curve analysis is more efficient, more convenient than direct sequencing. Moreover, it is a low-cost test, which is suitable for clinic test.
结论：新的高分辨率熔解曲线分析法在检测KRAS基因突变时比直接测序法具有耗时短，易操作的优点，并且检测成本低廉，适合在临床开展。
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