Sequencing analysis shows that the CpTI gene has been modified as what was DE - signed.
序列分析表明了基因修饰的准确性。
Ampicilin - resistant transformants were selected and identified by PCR, Enzyme digestion and DNA sequencing analysis.
并对氨苄筛选的重组入外源基因的质粒通过P CR、酶切和测序鉴定。
DNA sequencing analysis proved that the sequence ofthe radiation-inducible promoter was totally consistent with the designing sequence.
合成启动子序列分析与设计序列完全一致；
Conclusion MTC can be diagnosed at gene level by direct gene sequencing analysis. It is possible to diagnose MTC before operation by means of molecular genetic analysis.
结论直接基因测序分析能在基因水平诊断MTC，分子遗传学分析使术前诊断该疾病成为可能。
By PCR-SSCP analysis, 17 PCR products were identified with different mobility of single strand DNA in propositus. 9 suspectable mutations were revealed with DNA sequencing analysis.
SSCP分析发现17个外显子pcr产物单链dna迁移率异常，通过DNA直接测序发现9个外显子可疑突变，但反向测序均未能证实。
Additional laboratory analysis, including gene sequencing, is ongoing.
目前正在进行其它实验室分析，包括基因测序。
The technology for exome sequencing is also further along than for whole-genome sequencing, Wang added, including not only the sequencing, but the capture technologies and the data analysis.
外显子组测序技术也比全基因组测序走得更远，Wang补充道，不仅仅是测序，还包括捕获技术和数据分析。
Gene sequencing and homology analysis show that these novel enzymes share remarkable homology with the amylase family.
基因测序及同源性分析表明这些新酶与淀粉酶家族具有很强的同源性。
The popularity of rapid DNA sequencing technology provides large amounts of data for molecular phylogenetics, while the sequence analysis technology is the important tool to gain knowledge from data.
DNA快速测序技术的普及为分子系统学家提供了大量数据，而序列分析技术则是探索数据发现知识的重要工具。
After the completion of human genome sequencing, biologists desire for higher processing and analysis power to handle the huge gene data.
人类基因组测序工作完成后，对基因数据的处理和分析能力提出了更高的要求。
After the completion of human genome sequencing, the biologists require higher processing and analysis power to handle the huge gene data.
人类基因组测序工作完成后，对基因数据的处理和分析能力提出了更高的要求。
Methods We used heteroduplex mobility analysis (HMA) and sequencing to detect mixed infection.
方法使用异双体动态分析法和测序检测混合感染。
Advances in sequencing technologies pose challenges for high-throughput analysis of large-scale sequence data.
测序技术的进展为大规模序列数据的高通量分析提出了挑战。
Mutation analysis was detected by direct sequencing RT-PCR products.
直接测序法检测RT PCR产物的基因变异；
BACKGROUND: Due to the advanced techniques in sequencing and fragment analysis, DNA sequencers and analyzers produce vast amounts of data within short time.
背景：由于测序和片段分析中的先进技术，DNA测序仪和分析仪在短时间内就产生了大量的数据。
Simultaneously the computational analysis of the large volumes of data generated by the new sequencing machines remains a challenge.
同时，由新的测序机器产生的大量数据的计算分析仍然是一个挑战。
And identified HEV71 isolates were performed by gene amplification of VP1 coding region, nucleotide sequencing and homology analysis of evolution.
对分离到的HEV71阳性分离株进行VP1编码区基因扩增，核苷酸序列测定和同源进化分析。
However, at present, the analysis of next-generation sequencing data is challenging, in particular because most sequencing platforms provide short reads, which are difficult to align and assemble.
然而，目前，下一代测序数据的分析仍然面临挑战，尤其是大多数测序平台产生了短序列，这对比对和组装造成了困难。
The purified DNA can be used directly in subsequent enzymatic reactions such as labeling, sequencing, cloning, and ligation, as well as for PCR analysis.
纯化的DNA可直接用于后续的应用，如标记、测序、克隆、连接和PCR分析。
Specific primers were designed for MSX1 and PAX9 respectively. Mutation analysis was performed by direct sequencing of all the coding exons and intron-exon boundaries.
分别设计MSX1、PAX9基因特异性引物，聚合酶链反应扩增全部外显子编码区和内含子-外显子剪接序列，产物纯化后直接测序。
Analysis of RT-PCR and sequencing indicated that the ncRNA gene had two different transcripts through alternatively splicing pattern.
PCR和测序结果分析，发现这个非编码RNA基因通过选择性剪接产生两种不同的转录产物。
Mapping the sequencing reads to reference genomes efficiently and effectively is one of the most critical analysis tasks.
高效且有效地比对测序片段到参考基因组上是最关键的分析任务之一。
Conclusions: The new high resolution melting curve analysis is more efficient, more convenient than direct sequencing. Moreover, it is a low-cost test, which is suitable for clinic test.
结论：新的高分辨率熔解曲线分析法在检测KRAS基因突变时比直接测序法具有耗时短，易操作的优点，并且检测成本低廉，适合在临床开展。
ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis.
通过引物设计、P CR、基因克隆、测序，获得了10种鸢尾属植物和1个外类群种的ITS序列。
The manuscript describes the initial sequencing and analysis of rice chromosome 4.
这篇文章描述了水稻4号染色体的测序和分析工作。
Identification on pathogens of maize viral diseases in Shandong province was conducted by whole genomic sequencing, electron microscope observation, serological analysis and vector transmitting test.
利用基因组核苷酸全序列测定、电镜观察、血清学测定、传播介体传毒试验等方法对山东省田间发生的玉米病毒病进行了病原鉴定。
Methods:Various analytical techniques including gene sequencing, amino acid composition, analysis of N terminal amino acid analysis, peptide mapping, IEF, molecular weight and UV scanning were used.
方法：用基因测序、氨基酸组成分析、N末端氨基酸测序、胰肽图谱、IEF、分子量、紫外扫描等各种实验手段对重组L 天冬酰胺酶及其天然品进行了全面比较。
In this article, I review some of the conceptual issues and currently available software tools for the analysis of sequencing-based whole-genome epigenetics data.
本文中，我回顾了一个概念性问题及基于测序的全基因组表观遗传学数据分析的当前可用的软件工具。
Complete genome sequencing could characterize the Dengue virus isolate and the results could be used for the phylogenetic analysis with other viruses.
结论通过对登革病毒全基因组序列的测定可以获得毒株的特征。
Complete genome sequencing could characterize the Dengue virus isolate and the results could be used for the phylogenetic analysis with other viruses.
结论通过对登革病毒全基因组序列的测定可以获得毒株的特征。
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