Cloning and sequencing analysis of ORF2 gene of PCV2 Shaanxi isolate.
3.PCV2 ORF2基因的克隆及其序列分析。
参考来源 - 猪圆环病毒2型（PCV2）的研究Result:A DNA fragment of 1482bp was cloned and sequencing analysis indicated that it carried an entire open reading frame and encoded a protein of 493 amino acid residues.
结果:扩增了一个长1482bp的基因片断,序列分析表明其覆盖了完整编码框,编码由493个氨基酸组成的人TRIM5α基因。
参考来源 - 重组人TRIM5α基因嵌合体的构建、表达纯化及其体外抑制HIVOprD2 encoding gene sequencing analysis revealed that mutation were in all of strains.
19株细菌的OprD2编码基因测序分析结果显示Opr D2编码基因均发生小片段缺失。
参考来源 - 耐碳青霉烯类铜绿假单胞菌耐药机制研究Firstly, the 4.132kb 5'end regulatory region and the 1.805kb 3' end regulatory region were amplified from goat genome by the long fragment PCR technology. These two fragments were cloned into the pGEM-T easy vector for the sequencing analysis.
首先通过高保真长PCR从山羊基因组中扩增出4.132kb的5’端调控序列和1.805kb的3’端调控序列,将两片段克隆至pGEM-T easy载体上,进行部分序列测定,并进行同源性比较。
参考来源 - 山羊β·2,447,543篇论文数据，部分数据来源于NoteExpress
Sequencing analysis shows that the CpTI gene has been modified as what was DE - signed.
序列分析表明了基因修饰的准确性。
Ampicilin - resistant transformants were selected and identified by PCR, Enzyme digestion and DNA sequencing analysis.
并对氨苄筛选的重组入外源基因的质粒通过P CR、酶切和测序鉴定。
DNA sequencing analysis proved that the sequence ofthe radiation-inducible promoter was totally consistent with the designing sequence.
合成启动子序列分析与设计序列完全一致；
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