定点突变 site directed mutagenesis ; site-directed mutation ; oligonucleotide-directed mutagenesis ; Sequencing
基因定点突变 Gene-directed mutagenesis
定点突变试剂盒 site-directed mutagenesis kit
经由定点突变 Sit-directed mutagenesis
基因定点突变服务 genic mutation
PCR定点突变 PCR site-directed mutagenesis
dna定点突变 dna site-directed mutation
体外定点突变诱发技术 Site-specific in votro mutation
体外定点突变技术 PCR site-directed mutagenesis ; PCR-SDM
The above results suggested that codon usage optimization and site-directed mutagenesis technology could be utilized to improve the expression level of pro-transglutaminase in E. coli.
上述研究结果表明密码子优化及定点突变技术可以应用于优化转谷氨酰胺酶酶原在大肠杆菌中的表达。
参考来源 - 转谷氨酰胺酶酶原在大肠杆菌中的重组优化表达ConclusionsGene Furin K5 enzymatic hydrolysis of recognition sites for site-directed mutagenesis to enhance adenovirus-mediated expression of K5 protein secretion for further to adenovirus vector carrying the gene therapy K5 retinal neovascularization dysplasia diseases laid the foundation for experimental Study.
结论对K5基因Furin蛋白酶水解识别位点进行定点突变能提高腺病毒介导的K5蛋白分泌表达量,为进一步以腺病毒为载体携带K5基因治疗眼底新生血管异常增生性疾病的实验研究奠定了基础。
参考来源 - 携带Kringle5基因的重组腺病毒抗新生血管形成的实验研究In addition, the bioinformatics analysis, N-Glycosidase F treatment experiment, site-specific mutagenesis and cell aggregation assay were used to firstly demostrate that NECL1 is a glycoprotein with a single N-glycosylation site which can influence the cell-cell adhesion activity.
此外,我们还通过生物信息学分析、N-糖苷酶F(N-Glycosidase F)处理实验、定点突变、细胞粘附等方法首次证明了NECL1是一个具有单一N糖基化位点的糖蛋白,并表明其糖基化位点能影响细胞-细胞之间的相互作用。
参考来源 - NECL1蛋白在神经胶质瘤中表达缺失的表观遗传调控机制·2,447,543篇论文数据，部分数据来源于NoteExpress
本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
定点突变是研究基因功能的一种关键方法。
Site-directed mutagenesis is one of the key methods used to study gene function.
目的：介绍一种简便、有效的定点突变技术。
Objective: To develop a simple and efficient way to perform site-directed mutagenesis.
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