本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
着重阐述了基因定点突变技术、基因融合技术和翻译修饰技术等新兴定点固定化技术的原理、特点和操作。
The principles, operation and applications of site-directed mutagenesis, gene fusion technology, and post-translational modification methods were introduced emphatically.
定点突变是研究基因功能的一种关键方法。
Site-directed mutagenesis is one of the key methods used to study gene function.
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