本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
定点突变是研究基因功能的一种关键方法。
Site-directed mutagenesis is one of the key methods used to study gene function.
目的：介绍一种简便、有效的定点突变技术。
Objective: To develop a simple and efficient way to perform site-directed mutagenesis.
结果表明某些定点突变可以揭示影响酶活的重要功能域。
The results indicated that some site-directed mutagenesis can reveal important functional domains of enzyme activity.
用双引物法对GI基因进行体外定点突变，构建了GI突变体g 138p。
The mutant G138P was obtained by in vitro site directed mutagenesis of GI gene.
目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
大量定点突变实验、选择性化学修饰实验、抗多肽抗体结合实验等能很好地确证该联配结果的正确性。
The reliability of the method was confirmed by site-directed mutagenesis, selective chemical modification, site-specific antipeptide antibody binding experiments, and so on.
结论体外定点突变系统可以对基因进行精细的修饰，为建立更精确地模拟人类疾病的动物模型打下基础。
Conclusion the site specific point mutation system can modify human gene in vitro more accurately. It is useful in the setting up of animal models.
应用寡核苷酸诱导的定点突变和PCR技术对人脑源性神经营养因子基因进行突变，并完成了测序鉴定。
Human brain derived neurotrophic factor gene was used as template for oligonucleotide based site directed mutagenesis and PCR.
着重阐述了基因定点突变技术、基因融合技术和翻译修饰技术等新兴定点固定化技术的原理、特点和操作。
The principles, operation and applications of site-directed mutagenesis, gene fusion technology, and post-translational modification methods were introduced emphatically.
采用大引物方法，利用质粒多克隆位点两侧的普通测序引物作为旁侧引物，在单个PCR管内，经2个步骤共34个循环进行定点突变。
Using normal sequencing primers adjacent to multiple clone sites of plasmid as flanking primers, a novel megaprimer method was performed in one tube with two steps and 34 cycles.
方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；
Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.
定点基因突变以及单通道夹被用于探测爪蟾卵细胞中摇动钾离子通道被去活化时的分子转移过程。
Site-directed mutagenesis and single-channel patch-clamp recording were used to explore the molecular transitions that underlie inactivation in Shaker potassium channels expressed in Xenopus oocytes.
定点基因突变以及单通道夹被用于探测爪蟾卵细胞中摇动钾离子通道被去活化时的分子转移过程。
Site-directed mutagenesis and single-channel patch-clamp recording were used to explore the molecular transitions that underlie inactivation in Shaker potassium channels expressed in Xenopus oocytes.
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