This method solved the problem that the parts of sequencing ladders adjacent the primer could not be read with two-step protocol and labeling step was also omitted. The satisfactory sequencing ladders have been obtained by this improved "Klenow-type" protocol.
此法不但解决了二步法中引物后部分DNA顺序无法读出的缺点,而且简化了反应步骤,亦能得到令人满意的顺序分析结果,每次可读出至少400碱基的序列。
参考来源 - 利用Taq DNA聚合酶对PCR产物直接进行顺序分析·2,447,543篇论文数据，部分数据来源于NoteExpress
顺序分析使用统计建模来检测数据中的模式。
Sequential analysis USES statistical modeling to detect patterns in data.
顺序分析是一个两阶段的顺序模式挖掘流程1。
The sequence analysis is a two-stage process for sequential pattern mining 1.
核苷酸顺序分析表明存在1—3个核苷酸取代。
Sequence analysis revealed sequence differences of 1-3 nucleotides involving three separate codons.
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