酶切是对粘末端的DNA分子和载体分子进行切割,以获得相应的粘末端连接。酶切可以是单酶切也可以是双酶切。单酶切操作比较简单,双酶切困难。
酶切位点 GGATCC ; Restriction Enzyme cutting site ; CTCGAG ; GGCC
酶切分析窗口 Restriction Sites
双酶切反应 Double Digestion ; Double Digests ; digestion ; buffer digestion
外切酶 exonuclease ; exo-PG ; cbh ; Exoglycosidases
内切酶 endonuclease ; incision enzyme ; homing endonuclease ; Biolabs
部分酶切 Partial digestion
酶切图谱 Macrorestriction Map ; restriction map
限制酶切割 restriction enzyme digestion
限制酶切位点 restriction site
Results:1. Construct recombinant adenovirus expression vector successfully and confirmed correctby using enzyme cleavage.2.
结果:1、成功构建重组腺病毒载体,经酶切验证正确。
参考来源 - 带hTERT启动子的腺病毒干扰ERCC1基因表达逆转卵巢癌顺铂耐药的研究This study was conducted by PCR and digestion polymorphism based on tomato genome sequencing results.
利用番茄基因组测序结果、扩增片段多态性和酶切扩增多态性序列法,对候选基因进行染色体定位。
参考来源 - 基于潘那利番茄渐渗系发掘干旱胁迫响应基因及功能分析·2,447,543篇论文数据,部分数据来源于NoteExpress
通过pcr产物的酶切证实多态性情况。
Polymorphisms was identified by restriction analysis of PCR products.
用酶切和PCR的方法对重组质粒进行鉴定。
Used restrict enzyme and PCR to verify the reconstructed plasmid.
对胞壁质酶R2基因进行了限制酶切图谱分析。
The restriction map of R2 gene was construed for further use.
Or you could ask the question, I have this restriction enzyme, at what regions on this plasmid will it cut?
你也可以这样问,我的这种限制性内切酶,会从质粒的哪个区域切开呢
Cutting involves enzymes called restriction endonucleases or restriction enzymes, which I've already mentioned and they have names.
切割用到的酶叫限制性核酸内切酶,或限制性内切酶,我已经提到过了,而且它们都有名字
Here the unique thing was that there's a restriction site inside that is present in normal DNA and not present in sickle DNA.
刚才例子中,特性就是在正常的基因中,有酶切位点,而在患者的基因中却没有
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