...Japan)、BglⅡ(10 units/μl,TaKaRa,Japan)、EcoRI (15 units/ μl,TaKaRa,Japan)进行限制酶切割(restriction enzyme digestion):1μg 基因组DNA, 3μl 的10x restriction enzyme digestion buffer,3μl restriction enzyme(BamHI、BglII、 EcoRI),混匀后放入...
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如果不同的分子用相同的限制酶切割,从每个分子产生的末端都能互相结合,从而将两种不同的分子连接在一起。
If different molecules are cut with the same restriction enzymes, the ends produced at each one have the capacity to bind to each other, joining together the two different molecules.
锌指核酸酶是一种人工制做限制性内切酶,通过将锌指DNA结合区与限制性内切酶的DNA切割区融合获得。
Zinc finger nucleases (ZFNs) are artificial restriction enzymes made by fusing an engineered zinc finger DNA-binding domain to the DNA cleavage domain of a restriction enzyme.
Cutting involves enzymes called restriction endonucleases or restriction enzymes, which I've already mentioned and they have names.
切割用到的酶叫限制性核酸内切酶,或限制性内切酶,我已经提到过了,而且它们都有名字
In this case, this particular restriction enzyme cuts symmetrically like this, but not at the same point.
在我们这种情况下,这个限制性内切酶总是这样对称地切割,但两条链切割的位点不一样
If this was a sickle patient, so they had this gene instead it wouldn't get cut and when I went to look for that presence of that gene on this gel, it would appear as one large segment instead of a large one and a smaller one.
而如果是个镰状红细胞贫血的病人,他们的基因是不会被限制酶切割的,因此他们基因的凝胶电泳结果就不会呈现,一大一小的结果,而只有一个大的片段
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