结果:逆转录病毒载体能有效地将外源性基因导入血管内皮细胞并稳定表达。
Results: The CGRP gene could be effectively transferred into HUVEC and stably expressed.
结论成功构建了PIG - A基因逆转录病毒载体,感染突变型k562细胞后可以提高其CD 59的表达。
Conclusion the retrovirus vector containing the PIG-A gene was successfully constructed and the expression of CD59 on the mutant K562 cells could be increased.
目的构建含小鼠白细胞介素12双亚基及新霉素磷酸转移酶基因多顺反子逆转录病毒载体,并观察其在小鼠肝癌细胞中的表达。
Objective to construct a polycistronic retroviral vector containing both subunits cDNA of murine IL 12 and NeoR gene and investigate their expressions in murine hepatoma cells.
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