结果：逆转录病毒载体能有效地将外源性基因导入血管内皮细胞并稳定表达。
Results: The CGRP gene could be effectively transferred into HUVEC and stably expressed.
结论成功构建了PIG - A基因逆转录病毒载体，感染突变型k562细胞后可以提高其CD59的表达。
Conclusion the retrovirus vector containing the PIG-A gene was successfully constructed and the expression of CD59 on the mutant K562 cells could be increased.
目的构建含小鼠白细胞介素12双亚基及新霉素磷酸转移酶基因多顺反子逆转录病毒载体，并观察其在小鼠肝癌细胞中的表达。
Objective to construct a polycistronic retroviral vector containing both subunits cDNA of murine IL 12 and NeoR gene and investigate their expressions in murine hepatoma cells.
结果表明：在双基因逆转录病毒载体介导的小鼠骨髓细胞的基因转导中，IRES与内部SV40启动子相比，更能保证双基因的共同表达。
Results showed that IRES was superior to SV40 promoter for guarantee of genes co expression in bicistronic retrovirus mediated gene therapy.
结果表明：在双基因逆转录病毒载体介导的小鼠骨髓细胞的基因转导中，IRES与内部SV40启动子相比，更能保证双基因的共同表达。
Results showed that IRES was superior to SV40 promoter for guarantee of genes co expression in bicistronic retrovirus mediated gene therapy.
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