生物学中,基因工程的基本操作,表达载体(Expression vectors)就是在克隆载体基本骨架的基础上增加表达元件(如启动子、RBS、终止子等),使目的基因能够表达的载体。如表达载体pKK223-3是一个具有典型表达结构的大肠杆菌表达载体。其基本骨架为来自pBR322和pUC的质粒复制起点和氨苄青霉素抗性基因。
... general expression通式;一般式 expression vector表达载体 emotional expression表情;情绪表现 ...
基于2个网页-相关网页
真核表达载体 eukaryotic expression vector ; eukaryotic expression plasmid
原核表达载体 prokaryotic expression vector ; neurot rop hin ; Prokaryotic expression plasmid
重组真核表达载体 recombinant eukaryotic expression vector
植物表达载体 plant expression vector ; transient expression vector ; plant express vector
表达载体构建 vector construction
真核细胞表达载体 eukaryotic expression vector
慢病毒表达载体 lentiviral vector
分泌型表达载体 expression and secretion vector ; secrete-type expression vector ; secreted expression vector ; secretion express carrier
The correctly modified HVl gene was inserted into yeast expression vector pYC-DE-2 and identified.
序列正确的HV1基因与酵母α因子信号肽基因相连,一起插入酵母表达载体pYC-DE-2。
参考来源 - 水蛭素基因的合成及在酵母中表达与产物纯化的研究The LEA gene was obtained from Tamarix. sp, which is a drought resistance and salt tolerance plant. It was cloned irto the plant express vector pROK Ⅱ.
本研究是将从抗旱、耐盐植物柽柳(Tamarix.sp)中克隆得到的LEA基因构建到植物表达载体pROK Ⅱ上,并对烟草进行遗传转化,验证LEA基因的功能。
参考来源 - 晚期胚胎富集蛋白基因植物表达载体的构建及其功能验证Expression vector is one of the most important factors to prepare high expression cell clone.
表达载体系统是获得高表达抗体细胞株的关键。
参考来源 - 全抗体在CHO细胞的高效表达Results: The interfering eukaryotic expression plasmid of ADAM17 gene was transfected into the tumor cells successfully. Fluorescence microscopy showed that the transfection efficiency was about 80%.
结果:干扰真核表达载体成功转入四株肿瘤细胞,荧光显微镜观察其转染效率,其在24h时转染率高达80%。
参考来源 - RNAi沉默ADAMs家族基因对肿瘤细胞生物学活性的影响The results of study are as follows:1. Constructing express vector.
本研究主要成果如下:1.构建表达载体。
参考来源 - 早竹TB1基因克隆及拟南芥转基因体系的探索In this study,we choose the prp , that is related to accumulation of secondary wall material of cotton fiber cell, and E6 promoter, the promoter is of peculiar in cotton fiber cell, to constructer the plant expressed vector.
本论文选择同棉纤维细胞次生壁物质积累相关的prp基因和棉纤维特异性启动子E6启动子,构建植物表达载体。
参考来源 - prp基因改良彩棉纤维品质初步研究·2,447,543篇论文数据,部分数据来源于NoteExpress
构建了鹦鹉热衣原体主要外膜蛋白的表达载体。
An expressing vector of major outer-membrane protein of Chlamydia psittaci was constructed.
本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
目的研究含双启动子表达载体在真核细胞中的表达活性。
Objective To study the expressing activity of vector containing two promoters in eukaryotic cells.
The second issue is that the gene vector, in this case the plasmid, has to be compatible with the cells that you're trying to express the gene in.
接下来的问题是如何选择合适的基因载体,在这里用到的,质粒,必须与要用来表达基因的,受体细胞匹配
Then we're going to take these plasmid vectors and we're going to somehow put them in contact with cells in such a way that the cells ingest the DNA and they use it.
然后要使这些质粒载体,以一种特别的方式与细胞接触,让细胞吞入质粒载体并使之表达
Eventually what happens is that glucose transport molecules which are expressed and stored inside the cell get shuttled up to the surface, so the cells permeability to glucose goes up and more glucose can come in.
最终的结果是那些已经表达,并储存于细胞中的能够运输糖的载体分子,穿梭到细胞表面,这样细胞对糖的透过性提高,更多糖分子进入到细胞内部
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