原核表达载体,能携带插入的外源核酸序列进入原核细胞中进行表达的载体。
原核表达载体(prokaryotic expression vector) 大肠杆菌、 芽孢杆菌、 链霉菌表达载体 ⑴ 复制起始点; ⑵ 多克隆位点; ⑶ 抗生素抗性基因; ⑷ 启动子(P) 是启动外源基因表...
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...因子29 ;神经营养素 4 ;原核表达载体; [gap=1304]KEY WORDS :act ivi ty dependent neurot rop hic f act or29 ; neurot rop hin 4 ; p rokaryot ic exp ression vect or ..
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AIM:To construct a prokaryotic expression vector expressing a fully human bivalent anti-idiotype antibody and identify the expression product.
目的:构建全人源鼻咽癌双价抗独特型抗体原核表达载体,并对其产物作初步鉴定。
参考来源 - 人源鼻咽癌双价抗独特型抗体疫苗的制备及活性鉴定We successfully construct Procaryotic Expression vector pET 32a/SAP2、DMAL-c2x/SAP2,which can express soluble fusion protein when induced by IPTG under low temperature. Totally,we got 4mg target protein to be antigen for antibody checking in immunized animals through affinity chromatography and proteinase cleavage.
2.成功构建了pET 32a/SAP2、pMAL-c2x/SAP2原核表达载体,经IPTG低温诱导,可以表达出可溶性的融合蛋白,通过亲和层析及蛋白酶切得到了4mg目的蛋白作为抗原检测试验动物免疫后产生的抗体。
参考来源 - 白念珠菌天冬氨酸蛋白酶2真核表达载体的构建及原核表达The fused gene was cloned into the prokaryotic expression vector pET28a. The right readframe was confirmed by DNA sequence.
将此融合蛋白基因克隆至大肠杆菌原核表达载体pET28a中,序列测定证明其阅读框架正确。
参考来源 - A、O型口蹄疫双价疫苗研究·2,447,543篇论文数据,部分数据来源于NoteExpress
目的构建人胃动素受体基因的原核表达载体。
Objective to construct expression plasmid of human motilin receptor.
目的克隆人TRAIL基因,构建其原核表达载体。
To clone gene of the TRAIL and construct its prokaryotic expression vector.
目的构建结核杆菌分泌蛋白mpt53原核表达载体并进行表达和纯化。
Objective to construct a expression vector of MPT53 of mycobacterium tuberculosis and identify and purify the protein in the e.
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