目的观察磷酸盐对人胎盘碱性磷酸酶(HPAP)胍变性的影响，为酶活性部位的柔性理论提供证据。

Objective To study the effects of phosphate on guanidine denaturation of human placental alkaline phosphatase (HPAP) and provide a theoretical basis for flexibility hypothesis of enzyme active sites.

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方法应用分光光度法测定有磷酸盐及无磷酸盐存在时，HPAP在胍变性过程中的活力变化。

Methods Spectrophotometry was used to determine the changes of activity of HPAP during guanidine denaturation in the presence and absence of phosphate.

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