目的观察磷酸盐对人胎盘碱性磷酸酶(HPAP)胍变性的影响，为酶活性部位的柔性理论提供证据。
Objective To study the effects of phosphate on guanidine denaturation of human placental alkaline phosphatase (HPAP) and provide a theoretical basis for flexibility hypothesis of enzyme active sites.
方法应用分光光度法测定有磷酸盐及无磷酸盐存在时，HPAP在胍变性过程中的活力变化。
Methods Spectrophotometry was used to determine the changes of activity of HPAP during guanidine denaturation in the presence and absence of phosphate.
方法应用分光光度法测定有磷酸盐及无磷酸盐存在时，HPAP在胍变性过程中的活力变化。
Methods Spectrophotometry was used to determine the changes of activity of HPAP during guanidine denaturation in the presence and absence of phosphate.
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