首先是航空业，然后是道路和铁路运输，它们都从运送品种、地点和价格的严格限制中解放出来。

First airlines, then road hauliers and railways, were freed from restrictions on what they could carry, where they could haul it and what price they could charge.

youdao

如果她不能抑制这种性格，而你不也想终结这种友谊，就限制她谈论自己的时间；然后，给自己同样长的时间发表意见。

If she can't curb this trait and you don't want the friendship to end, limit how long you let her go on about herself; then give yourself equal air time.

youdao

然后，限制他（她）的状态报告的规模。

Then, limit the scale of his or her status reporting.

youdao

This gives you a biological mechanism for cutting, using restriction enzymes, and then you denature so that it falls apart, and then you renature so that it comes back together.

这是一种切割DNA的生物机制,用限制性内切酶,改变DNA的性质让它打开,然后让它合起来恢复它的性质

耶鲁公开课 - 生物医学工程探索课程节选

Well the first step would be to cut open the plasmid with a particular restriction enzyme, and then what if I take that same restriction enzyme and I cut up the DNA that I'm interested in.

第一步是用某种限制性内切酶把质粒切开,然后用同一种限制性内切酶,切出我想要的DNA

耶鲁公开课 - 生物医学工程探索课程节选

We're going to take a chromosomal DNA, we're going to digest it with this restriction enzyme, we're going to put it in this tube and run it on a gel, and we're going to see what results down here.

我们取一条染色体DNA,然后用限制酶进行分解,接着再放,在凝胶上进行电泳,之后看看结果如何

耶鲁公开课 - 生物医学工程探索课程节选