方法新鲜脐带内灌注胶原酶消化，获得内皮细胞，用含20%胎牛血清的M199液进行培养。
Methods Endothelial cells were acquired by filling collagen enzyme solution into the lumen of umbilical veins and then cultured in M199 with 20% fetal bovine serum.
新鲜脐带内灌注胶原酶消化，获得内皮细胞，用含20%胎牛血清的M199液进行培养。
Endothelial cells were acquired by filling collagen enzyme solution into the lumen of umbilical veins and then cultured in M199 with 20% fetal bovine serum.
比色法测定细胞培养液中超氧化物歧化酶(SOD)活性及细胞内丙二醛(MDA)含量。
The activity of superoxide dismutase (SOD) in cell culture solution and the content of malondialdehyde (MDA) in cell were determined with colorimetric method.
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