方法新鲜脐带内灌注胶原酶消化，获得内皮细胞，用含20%胎牛血清的M199液进行培养。
Methods Endothelial cells were acquired by filling collagen enzyme solution into the lumen of umbilical veins and then cultured in M199 with 20% fetal bovine serum.
新鲜脐带内灌注胶原酶消化，获得内皮细胞，用含20%胎牛血清的M199液进行培养。
Endothelial cells were acquired by filling collagen enzyme solution into the lumen of umbilical veins and then cultured in M199 with 20% fetal bovine serum.
比色法测定细胞培养液中超氧化物歧化酶(SOD)活性及细胞内丙二醛(MDA)含量。
The activity of superoxide dismutase (SOD) in cell culture solution and the content of malondialdehyde (MDA) in cell were determined with colorimetric method.
大豆幼苗经过培养处理形成三种内磷浓度，在两种不同营养液外磷水平下进行培养，研究大豆根系特征与磷素吸收利用之间的关系。
Soybean seedlings, which cultured for three internal phosphorus concentration, grown in solution culture for 5 weeks, were used to study the relationship between root character and P absorption.
方法利用盐酸利多卡因注射液联合震荡培养法分离纯化胎大鼠脑内小胶质细胞，免疫荧光技术及流式细胞技术鉴定小胶质细胞的纯度。
Method: Fetal rat microglia in brain was isolated and purified by lidocaine hydrochloride with shock culturing. The purification of microglia was detected by immunofluorescence and flow cytometry.
方法利用盐酸利多卡因注射液联合震荡培养法分离纯化胎大鼠脑内小胶质细胞，免疫荧光技术及流式细胞技术鉴定小胶质细胞的纯度。
Method: Fetal rat microglia in brain was isolated and purified by lidocaine hydrochloride with shock culturing. The purification of microglia was detected by immunofluorescence and flow cytometry.
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