色谱过程中携带待测组分向前移动的物质称为流动相。与固定相处于平衡状态、带动样品向前移动的另一相。
The radiochemistry purity can be improved to 99% by semi-HPLC weak acid mobile phase.
采用弱酸性流动相,半制备HPLC分离后产品的放射化学纯度达99%。
参考来源 - Aβ斑块显像剂:~(11)CSome separation on column C18 with P-cyclodextrin and derivatives as mobile phase additives were also carried out.3.
本研究还考察了手性分离中的一种模式:非手性固定相柱加入手性流动相添加剂——即β-环糊精类衍生物作为手性添加剂在C18柱上对部分氨基酸进行了拆分。
参考来源 - 纤维素类手性固定相的制备及协同效应研究·2,447,543篇论文数据,部分数据来源于NoteExpress
不同的检测器,对流动相的要求不同。
结果表明死体积与流动相种类、配比和柱温等因素有关。
Experimental results further showed that dead volumes were also related to factors, such as the characteristics of the mobile phase, its composition and the column temperature.
讨论了检测波长的选择和样品的制备,优化了流动相的组成。
The detection wavelength, sample preparation and the optimization of mobile phase constitution were discussed.
应用推荐