以乙腈-水（1：4）为流动相；
不同的检测器，对流动相的要求不同。
优化不同性质样品的提取条件及测定的流动相。
The conditions for extraction and mobile phase were optimized.
流动相为纯乙腈时，焓和熵对对映体的拆分均有贡献。
Additionally, enthalpy and entropy both contributed to enantioseparation when mobile phase was acetonitrile.
结果表明死体积与流动相种类、配比和柱温等因素有关。
Experimental results further showed that dead volumes were also related to factors, such as the characteristics of the mobile phase, its composition and the column temperature.
多相反应色谱，即多个流动相且伴随化学反应的色谱过程。
Multiphase reactive chromatography is a chromatographic process with multi-mobile phases and chemical reactions.
讨论了检测波长的选择和样品的制备，优化了流动相的组成。
The detection wavelength, sample preparation and the optimization of mobile phase constitution were discussed.
在一些情况下，为了改善峰形，样品溶液可以用流动相稀释。
In some cases, the sample may be diluted with mobile phase to improve the peakshape.
建立了配体交换色谱手性流动相法拆分茶氨酸对映体的方法。
Ligand exchange chromatography with chiral mobile phases was established for the separation of theanine enantiomers.
在吸附色谱中，流动相的溶质分子进行多次连续的吸附和脱附。
In adsorption chromatography, solute molecules of the mobile phase undergo many successive adsorptions and desorptions.
在不同的固定相和流动相条件下，对单糖的分离情况进行了分析。
With the different state phase and mobile phase, the separation of monosaccharide was researched.
流动相组成对这两种类型溶质的洗脱范围及洗脱能力的影响不同。
The composition of mobile phase has different influence on the elution range and ability to the two types of solutes.
重点讨论了流动相流速对分离度、峰容量和溶质洗脱顺序的影响。
It is mainly for the effect on the resolution, peak capacity and solute elution order.
目的：建立hplc手性流动相添加剂法拆分美托洛尔对映体的方法。
Objective: to establish an HPLC method for the resolution of metoprolol enantiomer using chiral mobile phase additives.
方法在线性放大模式下，使用重叠分辨图法对流动相组成进行优化调节。
Methods Under linear scale up mode, overlapping separation range map was used to optimize the composition of mobile phase.
它以纯有机溶剂作电色谱流动相，具有许多水溶液电色谱所不能比拟的优点。
There are many advantages which the ordinary CEC method doesnt have by using the pure organic solvent as mobile phase.
研究了流动相的选择，梯度淋洗程序的最佳化以及进样浓度与峰高的线性关系。
The selection of moving phase, gradient eluting procedure and line relation of sampling concentration with peak height were discussed.
采用乙酸乙酯处理样品，甲醇作流动相，测定了口香糖中抗氧化剂BHT含量。
The BHT in chewing gum was treated with ethyl acetate, then analyzed by high performance liquid chromatography with methanol as mobile phase.
结果表明被研究的手性农药戊唑醇在正丁醇作流动相极性改性剂时拆分效果较好。
The results indicate that the pesticide tebuconazole has a relatively good effect on enantiomeric separation and retention of tebuconazole.
同时，介绍了分析所用的色谱柱、流动相、使用的检测手段以及样品的提取方法；
The chromatographic columns, mobile phases as well as detecting devices and sampling devices used in HPLC for this purpose were described.
采用氨基键合硅胶柱，以乙腈-水(75∶25)为流动相，使用示差折光检测器。
NH2 column was used with the mobile phase of acetonitrile-water (75:25). The differential refractive index detector was used.
方法：固定其他色谱条件，改变流动相组成与配比，以分离结果的优劣优化流动相。
METHODS: Fixing other conditions than the composition and ratio of the mobile phase, estimate the optimum according to the separation results.
采用二元流动相程序洗脱高效液相色谱分离了蛋白质水解液中常见的18种氨基酸。
The separation of 18 amino acids in hydrolysis solution of proteins has been conducted by a binary gradient in HPLC.
继用氯仿-四氢呋喃-水(500：500：8)为流动相，梯度洗脱其他强保留组分。
Chloroform - tetrahydrofuran - water (500:500:8) was used as mobile phase for the gradient elution of other strongly retained substances.
结论本方法对流动相系统作了改进，方法简便、快速、准确，在降解产物较多时也适用。
CONCLUSION the mobile phase system was improved, So the method was convenient, rapid, accurate, precise and it was not influenced when there are the degradation products.
本文研究了以P 507萃淋树脂为固定相，盐酸为流动相的萃取色层法分离稀土元素。
In this work extractive chromatographic method is used for the separation of REE with P507-containing resin as stationary phase, and hydrochloric acid as mobile phase.
考察了流动相组成、溶质结构和柱温对对映体分离的影响，并初步探讨了手性识别的机理。
The effects of composition of mobile phase, the structure of solute and the column temperature on chiral separation were extensively studied. The chiral recognition mechanism was discussed.
以含乙腈的流动相作为洗脱剂，对茶饮料中微量色素物质的HPLC分析方法进行了研究。
Study on HPLC method for analyzing of trace pigments in ready-to-drink tea was conducted by using different eluants with acetonitrile as mobile phase.
甚至一些硅胶基质填料在某些情况下，遇到突然的流动相组成或者流速改变时都会发生变化。
Even the packing structure of silica-based packings shifted in some cases when the packings were exposed to sudden changes in solvent composition or flow rate.
甚至一些硅胶基质填料在某些情况下，遇到突然的流动相组成或者流速改变时都会发生变化。
Even the packing structure of silica-based packings shifted in some cases when the packings were exposed to sudden changes in solvent composition or flow rate.
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