你引用的规则是用于覆盖全范围梯度洗脱的（例如，有机溶剂从5改变到100%），并且所指的体积是以初始条件来重新平衡。
The rule that you quoted is intended to cover a full-range gradient (for example, 5–100% organic solvent) and refers to the amount of volume to reequilibrate at the initial conditions.
葡萄籽经有机溶剂梯度法及酶法提取，大孔树脂初步纯化，薄层色谱法分离纯化得到原花青素单体和二聚体。
The monomer and dimer of proanthocyanidins in grape seeds were obtained by gradient and enzymatic extraction, isolation with macroporous adsorption resin, and purification by TLC.
基于线性溶剂强度模型，应用特征线分析的方法求解梯度洗脱模式下的理想液相色谱模型。
The ideal model of liquid chromatography in gradient elution was solved by using the characteristic approach on the assumption of a linear solvent strength model.
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