你引用的规则是用于覆盖全范围梯度洗脱的（例如，有机溶剂从5改变到100%），并且所指的体积是以初始条件来重新平衡。
The rule that you quoted is intended to cover a full-range gradient (for example, 5–100% organic solvent) and refers to the amount of volume to reequilibrate at the initial conditions.
葡萄籽经有机溶剂梯度法及酶法提取，大孔树脂初步纯化，薄层色谱法分离纯化得到原花青素单体和二聚体。
The monomer and dimer of proanthocyanidins in grape seeds were obtained by gradient and enzymatic extraction, isolation with macroporous adsorption resin, and purification by TLC.
基于线性溶剂强度模型，应用特征线分析的方法求解梯度洗脱模式下的理想液相色谱模型。
The ideal model of liquid chromatography in gradient elution was solved by using the characteristic approach on the assumption of a linear solvent strength model.
本研究采用石油醚、乙醚、甲醇、水溶剂梯度提取法和多种柱层析法，对栽培橘黄裸伞子实体进行了化学研究。
The cultural fruiting bodies of the Gymnopilus spectabilis were phytochemical investigated using layer extracts with petroleum ether, diethyl ether, methanol and water and silica gel chromatography.
当含水溶剂通过预柱在线净化后，根据空白梯度色谱图可测试有机溶剂的纯度。
When the aqueous solvent (solvent a) is cleaned up in terms of an on-line precolumn, the purity of the organic solvent (solvent b) can be tested according to the chromatogram of the blank gradient.
当含水溶剂通过预柱在线净化后，根据空白梯度色谱图可测试有机溶剂的纯度。
When the aqueous solvent (solvent a) is cleaned up in terms of an on-line precolumn, the purity of the organic solvent (solvent b) can be tested according to the chromatogram of the blank gradient.
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