目的：MCPR 1是我室利用消减杂交技术克隆出来的新基因，本研究利用融合蛋白制备mcpr1的多克隆抗体。
Objective: To prepare polyclonal antibodies of MCPR1, which is a novel Mouse Cleft Palate Relate gene cloned by subtractive hybridization method at our department.
该技术平台的构建涉及到蛋白样品的制备、封闭液的选择、点样条件的选择、抗体的浓度及其杂交时间及数据的处理等多个方面。
This technology platform construction involves protein samples preparation, the choice of sealed liquid, the conditions of printing, concentration and hybrid time of antibody and data processing, etc.
采用杂交瘤技术制备人源性单克隆抗体存在许多困难，90年代初期出现了抗体库技术，为抗体的制备提供了新的途径。
But it is difficult to make humanized monoclonal antibody with hybridoma technique. Antibody library technique coming forth in the early 90s provides the new method to get the antibody.
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