目的：MCPR 1是我室利用消减杂交技术克隆出来的新基因，本研究利用融合蛋白制备mcpr1的多克隆抗体。
Objective: To prepare polyclonal antibodies of MCPR1, which is a novel Mouse Cleft Palate Relate gene cloned by subtractive hybridization method at our department.
该技术平台的构建涉及到蛋白样品的制备、封闭液的选择、点样条件的选择、抗体的浓度及其杂交时间及数据的处理等多个方面。
This technology platform construction involves protein samples preparation, the choice of sealed liquid, the conditions of printing, concentration and hybrid time of antibody and data processing, etc.
采用杂交瘤技术制备人源性单克隆抗体存在许多困难，90年代初期出现了抗体库技术，为抗体的制备提供了新的途径。
But it is difficult to make humanized monoclonal antibody with hybridoma technique. Antibody library technique coming forth in the early 90s provides the new method to get the antibody.
方法利用B细胞杂交瘤技术，建立分泌抗总黄曲霉毒素单克隆抗体杂交瘤细胞株。
Methods Hybridoma cell line excreting monoclonal antibody against total aflatoxins was produced by using B cell hybridoma technique.
建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞和RT-PCR克隆人抗体基因及噬菌体呈现技术构建人源抗体库的策略。
Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.
用杂交瘤技术获得了第二组抗人血小板的单克隆抗体。
We have obtained the second group of monoclonal antibodies against human platelet by means of hybridoma technique.
方法应用淋巴细胞杂交瘤技术，制备单克隆抗体，并对其进行鉴定。
Methods McAb were prepared and identified by applying the lymphocyte hybridoma technique.
方法应用淋巴细胞杂交瘤技术，制备单克隆抗体，并对其进行鉴定。
Methods McAb were prepared and identified by applying the lymphocyte hybridoma technique.
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