随后他递给塞缪尔手术钳，告诉他先把后腿之间的皮肤扯起来，使之与肌肉分离。
Then he handed Samuel the forceps and told him to pull up on the skin between the rear legs to separate it from the muscle.
方法采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。
Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.
方法实验采用的方法是全细胞膜片钳技术，实验材料为急性分离的牛蛙背根神经节神经元。
Method The experiment was carried out in freshly isolated bullfrog using a whole-cell patch-clamp technique.
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