随后他递给塞缪尔手术钳，告诉他先把后腿之间的皮肤扯起来，使之与肌肉分离。
Then he handed Samuel the forceps and told him to pull up on the skin between the rear legs to separate it from the muscle.
方法采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。
Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.
方法采用急性分离的小鼠心室肌细胞，低渗溶液激活容积敏感性氯通道电流，膜片钳全细胞记录法记录电流。
Methods Isolated mouse cardiac ventricular myocytes were subjected to whole-cell patch-clamp to record the hypotonicity activated chloride channel currents.
方法实验采用的方法是全细胞膜片钳技术，实验材料为急性分离的牛蛙背根神经节神经元。
Method The experiment was carried out in freshly isolated bullfrog using a whole-cell patch-clamp technique.
方法采用全细胞膜片钳技术在急性分离的大鼠背根神经节细胞上观察龙血素B对辣椒素诱发的辣椒素受体电流的影响。
Methods In acutely isolated rat DRG neurons, effects of loureirin B on capsaicin-evoked currents were observed using whole-cell patch-clamp technique.
目的：建立大鼠心肌细胞分离方法，用于膜片钳实验，并记录L型钙离子通道电流。
Objective: to devise a method to isolate calcium-tolerant cardiomyocytes from SD rats for the experiment of patch clamp, and record the currents of L-type calcium channels.
方法酶解法分离豚鼠单个心室肌细胞，应用全细胞膜片钳技术研究白藜芦醇对豚鼠单个心室肌细胞钾电流的影响。
Methods Enzyme digestion was performed to isolate single ventricular myocyte of guinea pig and whole-cell patch-clamp technique was used to record potassium current.
方法：用膜片钳实验技术记录胶原酶急性分离的豚鼠心室肌细胞钾通道电流。
Methods The whole cell patch clamp technique was used in isolated ventricular cells of guinea pigs.
笔者应用机械分离获得豚鼠单个初级精母细胞，采用全细胞式膜片钳技术记录该细胞离子通道电流。
Mechanical dissociation was used to obtain single primary spermatocytes from guinea pigs and iron channel currents in the primary spermatocytes were recorded by whole cell patch-clamp technique.
结论成功建立了一种简单快速的适用于膜片钳技术的大鼠皮层神经元急性分离改良方法。
Conclusion a simple and fast method for acutely isolating rats cortex neurons suitable for patch clamp is successfully found.
采用2把普通长直角钳及内镜外科器械钝性结合锐性分离膈神经及血管。
The nerve was mobilized with 2 common long right angle clamps and some endoscopic instruments by blunt and sharp dissection.
常规酶解法分离成年大鼠心室肌细胞，采用全细胞膜片钳技术观察U50，488H对钠电流的作用。
The change of sodium currents (INa) induced by U50,488H was detected by whole-cell recording mode using patch clamp.
表明本分离及原代培养方法，有益于大鼠海马锥体神经元生长，并适用于膜片钳记录。
The methods of isolation and primary cultivation of hippocampal pyramidal neurons of SD rats improved the growth of neurons and so might be used in patch-clamp recording.
用血管钳加手指钝性分离胎盘直至羊膜腔，破膜迅速娩出胎儿。
The amniotic membrane was ruptured immediately and the fetus was delivered rapidly.
采用全细胞电压钳记录技术，给予选择性受体阻断剂以分离不同的突触后受体电流。
The recordings of postsynaptic currents were performed by using the whole-cell voltage-clamp method.
方法：采用全细胞膜片钳记录法，在新鲜分离的大鼠背根神经节细胞上观察血竭对钠通道电流幅值的影响。
Method: the whole cell patch clamp recording technique was used in this study. Results: blood resin suppressed amplitude of the sodium channel currents in a dose dependent way.
急性分离单个的豚鼠气管平滑肌细胞，以膜片钳技术的内面向外式记录单通道电流。
Single channel currents were recorded by using the patch clamp technique in inside-out configuration.
急性分离单个的豚鼠气管平滑肌细胞，以膜片钳技术的内面向外式记录单通道电流。
Single channel currents were recorded by using the patch clamp technique in inside-out configuration.
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