In order to perform the secretory expression of Bacillus phytase and get better enzymatic characters because of the post-translation modification of yeast, Bacillus phytase phyC gene was expressed in yeast primarily.
为实现芽抱杆菌植酸酶助尹C基因的分泌表达，以及由酵母翻译后加工可能带来的酶学性质改善，首次进行了该基因在酵母中的表达研究。
参考来源 - 枯草芽孢杆菌植酸酶phyC基因的克隆及其表达研究P. pastoris has many of the advantages of higher eukaryotic expression systems such as high secretion expression, stable integration in the P. pastoris genomic gene, easy high density fermentation and production downstream purification and so on.
毕赤酵母(P. pastoris)表达系统是目前较完善的高效异源真核蛋白表达系统,具有高分泌表达、高稳定整合、易于高密度发酵和产物的下游纯化等优点。
参考来源 - 小麦过氧化物酶（WP1）在毕赤酵母中的表达、纯化及活性鉴定A model of expression and secretion of foreign proteins by plant tissue and root system were established, which provides some refferences for future fermentation with dustrialized production of foreign proteins.
该研究为后续微生物发酵生产和产业化以及进一步阐明外源蛋白分泌表达机理和建立植物根系分泌生物反应器提供依据。
参考来源 - 利用烟草和拟南芥建立BSFE生物反应器Two different signal peptides-vsi and gpp were used to investigate the secretory expression efficiency in S. lividans.
本工作应用两种不同信号肽—vsi和gPP对链霉菌的分泌表达进行研究。
参考来源 - 毒性T淋巴细胞相关抗原·2,447,543篇论文数据，部分数据来源于NoteExpress
为了进一步提高外源蛋白质的分泌表达，对信号肽序列进行了研究。
For further improving the secretion expression of heterogeneous proteins, in this research, the signal sequences were studied.
将胰岛素原基因融合到金色葡萄球菌蛋白a的基因上，构建成大肠杆菌中基因融合的外分泌表达载体。
The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein a.
结论构建了人瘦素的哺乳动物细胞表达载体，并成功地在COS 7细胞中获得重组人瘦素的分泌表达。
Conclusion Cloning and expression recombinants of human leptin are obtained and secretive recombinant human leptin is successfully expressed in COS-7 cells.
应用推荐