Conclusion DNA marker can be produced for laboratory use with RD-PCR technology, which is a simple, efficient and inexpensive method for experiment.
结论采用限制性差异显示技术制备DNA标记物，不仅操作简便、快捷、提高工作效益，且大大降低费用，更适合实验室实际需要。
Methods The gene fragment library of SH SY5Y cells was constructed with RD PCR technique.
方法采用RD P CR技术构建SH SY5Y细胞基因片段文库，并对每一个克隆进行测序。
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