Conclusion DNA marker can be produced for laboratory use with RD-PCR technology, which is a simple, efficient and inexpensive method for experiment.
结论采用限制性差异显示技术制备DNA标记物，不仅操作简便、快捷、提高工作效益，且大大降低费用，更适合实验室实际需要。
Methods The gene fragment library of SH SY5Y cells was constructed with RD PCR technique.
方法采用RD PCR技术构建SHSY5Y细胞基因片段文库，并对每一个克隆进行测序。
Methods The gene fragment library of SH SY5Y cells was constructed with RD PCR technique.
方法采用RD PCR技术构建SHSY5Y细胞基因片段文库，并对每一个克隆进行测序。
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