In certain methods, the lysate is heated for a time and at a temperature sufficient to denature proteins present in the lysate and cause their aggregation and precipitation.

在某些方法中，将所述溶胞产物在一定温度下加热一定时间，所述温度和时间足以使所述溶胞产物中存在的蛋白质变性并使其聚集和沉淀。

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In certain situations most of the methods in a class need to carry an annotation (such as the one in Version 4).

在某些情况下，类中的大多数方法需要携带注释(如版本4中所示)。

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For certain classes and methods in my application I want to see the invocation counts, the average self execution time and the average wall clock time.

对程序中的某些类和方法，我需要了解它们被调用次数，各自平均执行时间和整体平均时间

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While high-end evaluation methods like randomized control trials (RCTs) are the surest means of determining what interventions in fact cause certain outcomes, they are also time-consuming and costly and beyond the means of many, if not most, mainstream nonprofits.

FORBES: An Interview with Paul Carttar, Director of the Social Innovation Fund