In certain methods, the lysate is heated for a time and at a temperature sufficient to denature proteins present in the lysate and cause their aggregation and precipitation.

在某些方法中，将所述溶胞产物在一定温度下加热一定时间，所述温度和时间足以使所述溶胞产物中存在的蛋白质变性并使其聚集和沉淀。

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While high-end evaluation methods like randomized control trials (RCTs) are the surest means of determining what interventions in fact cause certain outcomes, they are also time-consuming and costly and beyond the means of many, if not most, mainstream nonprofits.

FORBES: An Interview with Paul Carttar, Director of the Social Innovation Fund