14 primers with stable amplification and rich polymorphism for ISSR were screened. The optimal annealing temperature for ISSR-PCR reaction was proposed by gradient PCR.
同时,根据最佳体系筛选扩增稳定、多态性丰富的ISSR引物,通过梯度PCR确定引物的最佳退火温度。
参考来源 - 紫椴天然种群遗传多样性和遗传分化的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
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