Result: Compared with normal PCR, Gradient PCR could identify the the optimal annealing temperature of aldosterone synthase gene.
结果：与普通pcr相比，梯度PCR能够迅速地确定CYP11B2基因的最佳退火温度。
Methods: Gradient PCR was employed in analysis of aldosterone synthase (CYP11B2) gene polymorphisms, and gel Imaging Technology was used to determine the optimal annealing temperature.
方法：采用梯度PCR对醛固酮合酶基因(CYP11B2)进行扩增，通过凝胶呈像技术判断CYP11 B2基因最佳退火温度。
Method the main influential factors were detected by PCR in serial concentration gradient and electrophoresis, then select the optimal experimental conditions.
方法对实验研究中的主要影响因素设置各系列浓度梯度，进行PCR反应后观察电泳结果选取最适条件。
Method the main influential factors were detected by PCR in serial concentration gradient and electrophoresis, then select the optimal experimental conditions.
方法对实验研究中的主要影响因素设置各系列浓度梯度，进行PCR反应后观察电泳结果选取最适条件。
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