...关键词 ： 基因，PDLIM4 ；启动子；报告载体；荧光素酶 [gap=2625]Keywords : Gene, PDLIM4; Promoter; Report gene vector; Luciferase ...
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Objective To construct saccharomyces cerevisiae expression vector with GFP as report gene.
目的构建以绿色荧光蛋白（GFP）为报告基因的酿酒酵母表达载体。
Objective To construct the retroviral(RV) vector with report gene enhanced green fluorescent protein(EGFP) and to explore the gene transfection efficiency of RV on SK-N-SH neuroblastoma cells.
目的构建携带报告基因增强型绿色荧光蛋白（EGFP）的反转录病毒载体，并且探讨病毒载体对SK-N-SH神经母细胞瘤细胞株的感染效率。
Results Saccharomyces cerevisiae expression vector with GFP as report gene was constructed and expressed successfully.
结果成功构建了以GFP为报告基因的酿酒酵母载体，并在酵母中得到表达。
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