Objective To construct saccharomyces cerevisiae expression vector with GFP as report gene.
目的构建以绿色荧光蛋白（GFP）为报告基因的酿酒酵母表达载体。
Objective To construct the retroviral(RV) vector with report gene enhanced green fluorescent protein(EGFP) and to explore the gene transfection efficiency of RV on SK-N-SH neuroblastoma cells.
目的构建携带报告基因增强型绿色荧光蛋白（EGFP）的反转录病毒载体，并且探讨病毒载体对SK-N-SH神经母细胞瘤细胞株的感染效率。
Results Saccharomyces cerevisiae expression vector with GFP as report gene was constructed and expressed successfully.
结果成功构建了以GFP为报告基因的酿酒酵母载体，并在酵母中得到表达。
Using Agrobacterium vacuum infiltration method the plant bivalent expression vector pBI121 containing GUS as a report gene was transformed into lettuce Lactuca sativa l.
以GUS基因作为报告基因，利用农杆菌真空渗透法，采取正交试验设计对莴苣LactucasativaL。
Using Agrobacterium vacuum infiltration method the plant bivalent expression vector pBI121 containing GUS as a report gene was transformed into lettuce Lactuca sativa l.
以GUS基因作为报告基因，利用农杆菌真空渗透法，采取正交试验设计对莴苣LactucasativaL。
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