这两种RT PCR的组合又被称之为“定量RT-PCR（quantitative RT-PCR）”RT-PCR技术相关试剂 oligo: 多聚体，相当于mRNA引物 AMV（M-MLV）：逆转录酶
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semi-quantitative rt-pcr 半定量rt ; 半定量 ; attacin基因
Real-time quantitative RT-PCR 反应 ; 实时定量PCR ; 实时定量RT ; 实时荧光定量RT
fluorescence quantitative RT-PCR 荧光定量RT
real-time fluorescence quantitative RT-PCR 实时荧光定量RT
Comparative quantitative RT-PCR 比较定量RT
Fluorescent Quantitative RT-PCR 荧光定量RT
relative-quantitative rt-pcr 相对定量rtpcr
real-time fluorescent quantitative RT-PCR 实时荧光定量RT
Florescent real-time quantitative RT-PCR 荧光实时定量PCR
Semi-quantitative RT-PCR analysis showed that the expression of CpGMP increased gradually with papaya fruit maturing and decreased when fruit began to soften.
半定量RT-PCR分析结果表明,CpGMP基因随着番木瓜果实成熟表达量逐渐增加,软化时又逐渐降低。
参考来源 - 番木瓜若干果实成熟相关基因克隆及遗传转化的研究Differential expression of selected genes was confirmed by Real-time quantitativeor semi quantitative RT-PCR.
选择部分显著差异表达基因行实时荧光定量或半定量RT-PCR鉴定。
参考来源 - 供体脾细胞联合抗CD154输注诱导小鼠肺移植慢性排斥反应免疫耐受基因表达谱研究The mRNA expression of c-jun and c-fos was examined by Quantitative RT-PCR at 0, 0.5,1,3,6 h after PH. The expression of cyclin D1 protein was detected by western blotting at 6 h,12 h after PH.
3.实时荧光定量PCR检测PH后0、0.5、1、3、6 h肝组织c-jun和c-fos mRNA表达；免疫印迹技术检测PH后6 h和12 h时细胞周期蛋白D1的蛋白表达。
参考来源 - 肠道胆汁酸缺乏对肝脏再生的影响及其机制研究·2,447,543篇论文数据，部分数据来源于NoteExpress
The results of semi-quantitative RT-PCR experiments supported the reliability of our microarray analysis.
半定量反转录-聚合酶链反应验证了芯片分析结果的可靠性。
Quantitative RT-PCR results indicated representation of OsBTB gene expression was corresponding with the selected resistant and pathogenesis-related genes.
定量PCR结果表明该基因的表达模式与所选取的抗性及抗性相关基因的表达模式一致。
System real time quantitative RT-PCR technology and immunohistochemistry were used to detect the Chlamydia trachomatis urogenital tract, the expression of UU.
结果：①实验组女性泌尿生殖道沙眼衣原体、解脲支原体的表达高于对照组。
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