The results of semi-quantitative RT-PCR experiments supported the reliability of our microarray analysis.
半定量反转录-聚合酶链反应验证了芯片分析结果的可靠性。
Quantitative RT-PCR results indicated representation of OsBTB gene expression was corresponding with the selected resistant and pathogenesis-related genes.
定量PCR结果表明该基因的表达模式与所选取的抗性及抗性相关基因的表达模式一致。
System real time quantitative RT-PCR technology and immunohistochemistry were used to detect the Chlamydia trachomatis urogenital tract, the expression of UU.
结果：①实验组女性泌尿生殖道沙眼衣原体、解脲支原体的表达高于对照组。
Results showed that 1. fluorescent quantitative RT-PCR was an efficient quantification method to detect the gene expression level and has a prospecting application in clinic;
结果显示，1．荧光定量RT干CR方法是一种定量检测基因表达丰度的有效方法，具有很好的临床应用前景；
Results : Detection of MTHFR gene C677T polymorphism was quickly finished by realtime fluorescence quantitative RT - PCR, and the results were proved to be effective by PCR - RFLP.
结果：荧光定量方法可以快速完成MTHFR C677T基因多态性的检测，检测结果得到传统PCR-RFLP方法的证实。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
The gene expression of ET-1, ETAR, ETBR and ECE was evaluated by semi-quantitative reverse transcription polymerase chain response (RT-PCR).
采用半定量逆转录多聚酶链反应(RT - PCR)检测局部内皮素系统ET - 1、ETAR、ETBR及ECE的基因表达。
Objective To increase the sensitivity of quantitative detection of gene transcripts in peripheral blood using real time RT PCR, two specimen processing methods were compared.
目的选择合理的标本处理方法，提高外周血基因转录本检测的灵敏度。
Quantitative RT PCR method were used to detect the mRNA levels of multidrug resistance gene in 32 leukemia children.
应用逆转录PCR结合同位素定量分析，对32例儿童白血病患者的多源耐药基因表达水平进行了研究。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
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