目的构建携带双自杀基因且可诱导敲除SV40T的逆转录病毒载体，优化目前的肝细胞永生化。
Objective To construct a new retroviral vector with double-suicide genes and knockout-inducible SV40T gene, with the aim of optimizing immortalization of hepatocytes.
结论构建了含hpv16e7的逆转录病毒载体，为人关节软骨细胞的基因转染打下了基础。
Conclusion Successfully constructed retroviral vector encoding for HPV16E7 establishes important basis for gene transfection of human articular chondrocytes.
构建并鉴定携带人NT - 3基因的逆转录病毒载体以及稳定的包装细胞系。
Objective:To construct a recombinant defective retroviral vector carrying hNT3 gene and a stable virus-producing packaging cell lines , and then to identify them.
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