目的构建携带双自杀基因且可诱导敲除SV40T的逆转录病毒载体，优化目前的肝细胞永生化。
Objective To construct a new retroviral vector with double-suicide genes and knockout-inducible SV40T gene, with the aim of optimizing immortalization of hepatocytes.
结论构建了含hpv16e7的逆转录病毒载体，为人关节软骨细胞的基因转染打下了基础。
Conclusion Successfully constructed retroviral vector encoding for HPV16E7 establishes important basis for gene transfection of human articular chondrocytes.
构建并鉴定携带人NT - 3基因的逆转录病毒载体以及稳定的包装细胞系。
Objective:To construct a recombinant defective retroviral vector carrying hNT3 gene and a stable virus-producing packaging cell lines , and then to identify them.
结论成功构建了PIG - A基因逆转录病毒载体，感染突变型k562细胞后可以提高其CD59的表达。
Conclusion the retrovirus vector containing the PIG-A gene was successfully constructed and the expression of CD59 on the mutant K562 cells could be increased.
目的构建含小鼠白细胞介素12双亚基及新霉素磷酸转移酶基因多顺反子逆转录病毒载体，并观察其在小鼠肝癌细胞中的表达。
Objective to construct a polycistronic retroviral vector containing both subunits cDNA of murine IL 12 and NeoR gene and investigate their expressions in murine hepatoma cells.
目的构建反义及正义波形蛋白重组逆转录病毒载体，研究波形蛋白在反应性胶质化中的功能与作用。
Objective to construct antisense and sense vimentin retrovirus vector for investigating the effect of vimentin on reactive gliosis.
方法构建克隆1个含有JAK2基因和二聚化化学诱导物(AP2 0 187)结合位点所组成的逆转录病毒载体。
Methods a retrovirus vector (RV) which contains JAK2 gene and two binding sites for a chemical inducers of dimerization (AP20187) was constructed.
方法构建克隆1个含有JAK2基因和二聚化化学诱导物(AP2 0 187)结合位点所组成的逆转录病毒载体。
Methods a retrovirus vector (RV) which contains JAK2 gene and two binding sites for a chemical inducers of dimerization (AP20187) was constructed.
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