共生食用菌松茸菌丝体液体发酵的研究 关键词：松茸；菌丝体；种龄；补料培养；液体发酵 [gap=1610]Key words：Tricholoma matsutake; mycelium; inoculum age; feeding culture; submerged culture
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In fed-batch culture, the best fed volum was 60% of the working volum, the best time was peak time of hydrogen production, in above conditions, YUAN-3 kept higher growth and hydrogen-producing activity.
补料培养的最佳补料体积为发酵液体积的60%左右,最佳的补料时间为产气的高峰期,在此条件下投加细菌可以保证细菌具有较高的生长和产氢活性,并可以缩短延滞期。 连续培养能够不断将发酵产物排出,引进新鲜培养基,避免底物的抑制。
参考来源 - 自凝集产氢细菌代谢特性及扩大培养研究·2,447,543篇论文数据，部分数据来源于NoteExpress
实验还表明，分批补料培养可以提高菌体浓度，可使转硒效率和绝对量增加。
We also found that the fed-batch can enhance the concentration of thalli and increase the efficiency and the absolute value of transferring se.
本文研究了玉米皮水解液发酵生产饲料酵母的摇瓶间歇培养和分批补料培养条件，进行了10L反应器分批补料培养试验。
The biotechnology of flask batch and fed-batch culture to produce yeast from cornhusk hydrolysate was studied and the fed -batch process in 10L bioreactor was conducted.
实现了液态培养基的连续补料和代谢产物的连续分离。
It can realize the dynamic equilibrium of culture medium and production .
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