荧光定量通过荧光染料或荧光标记的特异性的探针，对PCR产物进行标记跟踪，实时在线监控反应过程，结合相应的软件可以对产物进行分析，计算待测样品模板的初始浓度。
【摘要】： 本研究利用Solexa测序技术并结合生物信息学和荧光定量(qRT-PCR)分析,首次从正茬(种植1年)地黄和重茬(种植2年)地黄中鉴定了82个保守的miRNAs(属于24个家族)和7个新型的miRNAs(属于7个家族),预测...
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...荧光定量； 阈值 中图分类号： TS252．7 文献标识码： A 文章编号： 1001- 2230（ 2008） 07- 0047- 03 [gap=980]Key words： E． sakazakii； Fluorescent quantitative； cycle threshold ..
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实时荧光定量PCR Real-time PCR ; real-time fluorescent quantitative PCR ; real-time quantitative PCR ; Real-time quantitative reverse transcription-PCR
荧光定量PCR仪 real-time qPCR ; fluorescence ration PCR instrument ; Real time PCR ; Fluorescent Quantatitive PCR
荧光定量聚合酶链反应 fluorescence quantitative polymerase chain reaction ; FQ-PCR ; FQ ; fluorescence quantitative PCR
实时荧光定量RT quantitative real-time RT-PCR ; real-time fluorescence quantitative RT-PCR
荧光定量RT-PCR fluorescence quantitative RT-PCR ; Real time quantitative RT-PCR ; Real-time RT-PCR ; quantitative rt-pcr
实时荧光定量 Real-time quantitative ; PCR ; Real-time RT-PCR
荧光定量PCR技术 real-time fluorescent quantitative PCR ; fluorescent quantitative PCR
时荧光定量PCR仪 real-time qPCR
荧光定量检测 Fluorogenic quantitative detection
Objective To develop a real-time fluorescent quantitative PCR method for detection of human telomerase reverse transcriptase(hTERT)gene in the plasma of patients with liver cancer.
目的建立实时荧光定量PCR法检测肝癌患者血浆端粒酶逆转录酶(Human telomerase reverse transcriptase,hTERT)基因水平。
参考来源 - 肝癌患者血浆端粒酶逆转录酶基因实时荧光定量PCR检测方法的建立Method Using the two-step real-time fluorescence quantitative reverse transcription polymerase chain reaction to detect PPARγ2 and UCP2 mRNA expression in adipose and liver tissue.
方法:两步法实时荧光定量逆转录聚合酶链反应检测脂肪组织和肝脏组织PPARγ2、UCP2 mRNA表达。
参考来源 - 肥胖与肥胖抵抗型大鼠非酒精性脂肪肝实验研究及βMETHODS: Fluorescent quantitation PCR was used to detect the MACC1 and c-MET mRNA in 67 cases of renal carcinoma and peri-cancerous normal tissues.
方法:应用荧光定量PCR检测67例肾癌及相应癌旁正常组织中MACC1和c-MET mRNA的表达。
参考来源 - 肾癌细胞增殖过程MACC1和cIn terms of telomerase activity, the level of hTERT (human telomerase reverse transcriptase) mRNA was assayed by FQ-PCR (fluorescence quantitative polymerase chain reaction) to reflect telomerase activity.
在对端粒酶活性的影响方面,采用荧光定量PCR法,检测端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)的mRNA水平来间接反映端粒酶的活性。
参考来源 - 新藤黄酸抗肿瘤作用及其机制研究The expression levels of flavone synthaseⅡwere analyzed in tea germplasms using Real-Time PCR technology. The FSⅡgene expressed in mature leaves, spring‘two and a bud’, seeds, petals, stamen and pistils of Longjing 43.
2.利用荧光定量PCR技术分析茶树种质资源中FSⅡ基因的表达情况。
参考来源 - 茶树黄酮合成酶Ⅱ基因的克隆、生物信息学与荧光定量PCR分析Real-time quantitative PCR (RtPCR) is regarded as the most accurate and sensitive methods for rapidly detecting pathogens in various samples.
实时荧光定量PCR被认为是目前检测病原体的最快速的高特异和高敏感的新技术,已开始用于病毒、细菌等病原体的快速检测。
参考来源 - 荧光定量PCR检测立克次体·2,447,543篇论文数据，部分数据来源于NoteExpress
结果建立的实时荧光定量PCR方法的灵敏度为10 -4水平。
Results The sensitivity of the established real time quantitative PCR was at 10 -4 level.
构建汉坦病毒S片段标准品，用于实时荧光定量pcr检测汉坦病毒。
To explore the method of preparation for Hantavirus s segment standard plasmids of real-time PCR.
目的探讨荧光定量聚合酶链反应（FQ-PCR）技术诊断结核病临床应用价值。
Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis.
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