实时荧光定量PCR (Real-time PCR)技术于1996年由美国Applied Biosystems公司推出,由于该技术不仅实现了PCR从定性到定量的飞跃,而且与常规PCR相比,它具有特异性更强、有效解决PCR污染问题、自动化程度高等特点,目前已得到广泛应用。本文试就其定量原理、方法及参照问题作一介绍。
实时荧光定量PCR(Real-time PCR)即通过荧光染料或荧光标记的特异性探针对 PCR反应过程进行实时监测,根据PCR指数扩增期间荧光信号的变化来即时测定对应特异产物的...
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实时荧光定量PCR仪 Real-time PCR
实时荧光定量 PCR qRT-PCR ; real-time quantitative PCR ; real-time PCR
荧光实时定量PCR Real time quantitative PCR ; quantitative real time PCR ; Florescent real-time quantitative RT-PCR ; replication
实时定量荧光PCR real-time quantitative PCR
荧光实时定量pcr real-time pcr
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