类原球茎,为胚胎发生与器官发育的复合体,是植物在组培条件下产生的人工种子,在植物发育生物学、植株高效再生、遗传转化、次生代谢物质生产、细胞工程等研究领域均有广泛的应用前景。
研究表明:基本培养基的类型和继代时间直接影响蝴蝶兰类原球茎(Protocorm-like-body,PLB)的增殖速率,在改良狩野和改良KC培养基中类原球茎的增殖速率明显高于MS培养基;对培养2.5个月的类原...
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...ULTURAL UNIVERSITY》-2005 1X(2005)03-0060-04 通过正交试验,筛选了金钗石斛Dendrobium nobile类原球茎(protocrom-like bodies, PLBs)诱导及增殖适宜的培养基.
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类原球茎外植体 PLB explant
This research studied on the tissue culture technique and rapid propagation of Phalaenopsis PLB (protocorm-like body) , and the influence of flowering at different N, P, K level and using of different exogenous hormones.
本课题研究了蝴蝶兰组织培养类原球茎快繁技术,以及氮、磷、钾水平和激素施用对蝴蝶兰开花的影响。
参考来源 - 蝴蝶兰组织培养与花期调控技术研究Improved MS culture medium was chosen to investigate the effects of rare earth elements on cell growth, accumulation of polysaccharides, utilization of nutrient together with phosphorus and pH in medium from protocorm-like bodies (PLBs) of Dendrobium huoshanense in this paper.
本实验以改良的MS培养基为基木培养基,考察了稀土元素对霍山石斛类原球茎生长、多糖合成、碳氮磷代谢以及培养基pH的影响。
参考来源 - 稀土元素对霍山石斛类原球茎生长和多糖合成影响的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
类原球茎诱导分化过程中所需的蔗糖浓度相对较低。
Low level of sucrose is required during induction of Protocorm Like-Body and shoots differentiation.
不同浓度秋水仙素诱变时间长短与类原球茎细胞内部结构发生变化的程度关系密切;
The time of shoot formation was longer and the rate of shoot formation was lower when the concentration of colchicines got higher or the time of treatment got longer.
取继代培养30 d的铁皮石斛类原球茎作接种材料,以N6为基本培养基,加入植物激素,并以真菌提取物作诱导子,对类原球茎和愈伤组织进行诱导培养。
Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants, N6 was used as the basic culture with phytohormone added, and fungal extracts as the elicitor.
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