类原球茎诱导分化过程中所需的蔗糖浓度相对较低。
Low level of sucrose is required during induction of Protocorm Like-Body and shoots differentiation.
不同浓度秋水仙素诱变时间长短与类原球茎细胞内部结构发生变化的程度关系密切；
The time of shoot formation was longer and the rate of shoot formation was lower when the concentration of colchicines got higher or the time of treatment got longer.
取继代培养30 d的铁皮石斛类原球茎作接种材料，以N6为基本培养基，加入植物激素，并以真菌提取物作诱导子，对类原球茎和愈伤组织进行诱导培养。
Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants, N6 was used as the basic culture with phytohormone added, and fungal extracts as the elicitor.
取继代培养30 d的铁皮石斛类原球茎作接种材料，以N6为基本培养基，加入植物激素，并以真菌提取物作诱导子，对类原球茎和愈伤组织进行诱导培养。
Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants, N6 was used as the basic culture with phytohormone added, and fungal extracts as the elicitor.
应用推荐