猪卵母细胞的体外成熟培养、成熟卵母细胞的孤雌激活和胚胎培养是胚胎生物工程技术的主要环节。
The culture of porcine oocytes maturation in vitro, parthenogenetic activation of MII porcine oocytes and embryo culture are main technology of embryo biology engineering.
结果表明，猪卵丘细胞能促进小鼠卵母细胞的体外成熟，但并不影响小鼠卵母细胞的受精和胚胎发育。
The results showed that porcine cumulus cells could enhance maturation of mouse oocytes in vitro but not affect in vitro the fertilization of the oocytes and subsequent embryo development.
结论双相接种法是一种高效的组织工程骨构建方法，有利于提高接种效率和促进组织工程骨的体外成熟。
Conclusions Biphasic seeding is an effective method for fabricating tissue-engineered bone, can improve the seeding efficiency and facilitate in vitro maturation of the tissue-engineered bone.
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