3.1 猪卵母细胞的体外成熟(In vitro maturation, IVM) 从屠宰场采集初情期前母猪卵巢，置于含双抗 的30℃生理盐水中，并在2 h 内运回实验室。
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猪卵母细胞的体外成熟培养、成熟卵母细胞的孤雌激活和胚胎培养是胚胎生物工程技术的主要环节。
The culture of porcine oocytes maturation in vitro, parthenogenetic activation of MII porcine oocytes and embryo culture are main technology of embryo biology engineering.
结果表明，猪卵丘细胞能促进小鼠卵母细胞的体外成熟，但并不影响小鼠卵母细胞的受精和胚胎发育。
The results showed that porcine cumulus cells could enhance maturation of mouse oocytes in vitro but not affect in vitro the fertilization of the oocytes and subsequent embryo development.
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