哺乳动物细胞表达载体 Mammalian cell expression vector
目的:优化哺乳动物细胞表达系统,提高目的基因的表达效率。
Objective: to increase the expression level of foreign gene by the optimization of the expressional regulatory elements in the cassette of interesting gene.
结论构建了人瘦素的哺乳动物细胞表达载体,并成功地在COS 7细胞中获得重组人瘦素的分泌表达。
Conclusion Cloning and expression recombinants of human leptin are obtained and secretive recombinant human leptin is successfully expressed in COS-7 cells.
在哺乳动物细胞里,启动子是一个包含着许多蛋白质结合位点的复杂区域,它调节基因的表达。
In mammalian cells, the promoter is a complex region containing binding sites for many proteins that regulate gene expression.
One way that that's done is by introducing genes into cancer cells, genes that aren't normally expressed in any mammalian cell, but that will cause the cancer cell to die.
已经成功应用的一种方法是,将基因导入癌细胞,而这种基因通常不在哺乳动物细胞中表达,但是可以导致癌细胞的死亡
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